Takanari Tanabata @ National Institute of Agrobiological Science
|Measured variables||length, width, perimeter|
|Plant requirements||any, seeds|
SmartGrain: High-Throughput Phenotyping Software for Measuring Seed Shape through Image AnalysisT. Tanabata, T. Shibaya, K. Hori, K. Ebana, M. YanoPLANT PHYSIOLOGY, 2012 View paper
SmartGrain is a software for high-throughput measurement of seed shape. This software uses a new image analysis method to reduce the time taken in the preparation of seeds and in image capture. Outlines of seeds are automatically recognized from digital images, and several shape parameters, such as seed length, width, area, and perimeter length, are calculated. SmartGrain removed areas of awns and pedicels automatically. High-throughput measurement with SmartGrain reduced sampling error and made it possible to distinguish between lines with small differences in seed shape. SmartGrain could accurately recognize seed not only of rice but also of several other species, including Arabidopsis (Arabidopsis thaliana). The software is free to researchers.
Source: Tanabata et al. (2012) Plant Physiology , 160(4), 1871-80