Plant Image Analysis Plant Image Analysis

RootScape

Coruzzi lab @ New York University
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Scientific article
RootScape: a landmark-based system for rapid screening of root architecture in Arabidopsis.
D. Ristova, U. Rosas, G. Krouk, S. Ruffel, KD. Birnbaum, GM. Coruzzi,
2013 , Plant Physiol. , 161(3), 1086
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Description

RootScape is a landmark-based allometric method for rapid phenotyping of Root System Architecture. Using the software AAMToolbox, we created a 20- point landmark model that captures RSA as one integrated trait, and used this model to quantify changes in the RSA of Arabidopsis (Col-0) wild-type plants grown under different hormone treatments. Principal Component Analysis (PCA) was used to compare RootScape to conventional methods designed to measure root architecture. This analysis showed that RootScape efficiently captured nearly all the variation in root architecture detected by measuring individual root traits, and is five-to-ten times faster than conventional scoring.

RootScape was validated by quantifying the plasticity of RSA in several mutant lines affected in hormone signaling. The RootScape analysis recapitulated previous results that described complex phenotypes in the mutants and identified novel gene-by-environment interactions.



Source: Ristovaet al. (2013) Plant Physiology , 161(3), 1086-96

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Overview
Operating system
windows, mac, linux,

Licence
freeware, matlab,

Measured variables
shape,

Automation level
semi-automated,

Plant requirements
arabidopsis,

Image requirements
gif, tiff, jpg, png,

Export formats
csv,

Other information
-

Additional information

Install and use RootScape:

For more information about the RootScape software, please refer to the Coruzzi’s lab webpage