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FibrilTool, an ImageJ plug-in to quantify fibrillar structures in raw microscopy imagesArezki Boudaoud, Agata Burian, Dorota Borowska-Wykręt, Magalie Uyttewaal, Roman Wrzalik, Dorota Kwiatkowska, Olivier HamantNature Protocols, 2017 View paper
FibrilTool is an ImageJ plug-in based on the concept of nematic tensor, which can provide a quantitative description of the anisotropy of fiber arrays and their average orientation in cells, directly from raw images obtained by any form of microscopy. FibrilTool has been validated on microtubules, actin and cellulose microfibrils, but it may also help analyze other fibrillar structures, such as collagen, or the texture of various materials. The tool is ImageJ-based, and it is therefore freely accessible to the scientific community and does not require specific computational setup. The tool provides the average orientation and anisotropy of fiber arrays in a given region of interest (ROI) in a few seconds.
Source: Nature Protocole